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目的探讨补阳还五汤对局灶性脑缺血大鼠神经元再生的影响。方法Wistar大鼠随机分为假手术组、模型组、补阳还五汤组,每组32只,模型组、补阳还五汤组采用大脑中动脉阻塞法复制脑缺血模型,假手术组仅切开皮肤、分离右侧颈总动脉即缝合,补阳还五汤组在造模后予以补阳还五汤灌服,每日一次;另设正常对照组8只,不做任何处理。大鼠于实验开始后第一周每日腹腔给予5-溴脱氧核苷一次,此后每周两次。分别于1、7、14、28d时各处死8只大鼠(正常对照组为2只),观察大鼠神经功能,采用免疫组织化学法检测新生神经元数。结果补阳还五汤能显著减轻神经功能缺损;缺血后每组大鼠脑内均有少量新生神经元,补阳还五汤组数量较多,在7、14、28d与模型组比较,差异有显著性(P<0.05或P<0.01)。结论缺血后大鼠脑内存在有限的神经再生,补阳还五汤能增强此增生。
Objective To investigate the effect of Buyang Huanwu decoction on neuronal regeneration in rats with focal cerebral ischemia. Methods Wistar rats were randomly divided into sham operation group, model group, and Buyang Huanwu Decoction group. There were 32 rats in each group. The model group and Buyang Huanwu Decoction group used cerebral artery occlusion to replicate cerebral ischemia model. Only the skin was incised and the right common carotid artery was dissected and sutured. The Buyang Huanwu Decoction group was fed with Buyang Huanwu Decoction once a day after the model was established, and another 8 normal control groups were made without any treatment. Rats were given intraperitoneal 5-bromodeoxynucleotide once a day for the first week after the start of the experiment, and thereafter twice a week thereafter. Eight rats (2 in the normal control group) were sacrificed at 1, 7, 14 and 28 days respectively. The neurological function of the rats was observed. The number of newborn neurons was detected by immunohistochemistry. Results Buyang Huanwu Decoction could significantly reduce the neurological deficits. After the ischemia, there was a small amount of new neurons in the brain of each group. The number of Buyang Huanwu Decoction was more than that of the model group on the 7th, 14th, and 28th days after ischemia. The difference was significant (P<0.05 or P<0.01). Conclusion There is limited nerve regeneration in the rat brain after ischemia, and Buyang Huanwu Decoction can enhance this hyperplasia.