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目的:探讨癌基因C-erbB-2在周围型肺癌发生、发展中的作用。方法:采用免疫组化和流式细胞术、聚合酶链反应三种方法检测基因表达是否一致及与生物学行为、CT征象的关系。结果:癌基因C-erbB-2蛋白表达与患者的年龄、性别、TNM分期无关,在肺腺癌中的表达明显高于鳞癌(P<0.05),肺癌的分化程度低、有肺门或纵隔淋巴结转移C-erbB-2蛋白表达明显增高(P<0.05)。C-erbB-2蛋白表达与深分叶征、棘突征有关;而与肿瘤的大小、短细毛刺征、胸膜凹陷征无关。三种方法检测C-erbB-2蛋白及mRNA表达水平一致。结论:癌基因C-erbB-2在肺癌发生、发展、CT征象中起重要作用,检测这种基因并结合CT征象可作为临床诊断及评估预后的有效指标。
Objective: To investigate the role of oncogene C-erbB-2 in the occurrence and development of peripheral lung cancer. Methods: Three methods of immunohistochemistry, flow cytometry and polymerase chain reaction were used to detect whether the gene expression was consistent with the biological behavior and CT signs. Results: The expression of oncogene C-erbB-2 was not related to age, sex and TNM stage in patients with lung adenocarcinoma. The expression of C-erbB-2 protein in lung adenocarcinoma was significantly higher than that in squamous cell carcinoma (P <0.05) The expression of C-erbB-2 in mediastinal lymph node was significantly increased (P <0.05). The expression of C-erbB-2 protein was related to deep lobulation and spine signs, but not to tumor size, short spiculation and pleural indentation. Three methods to detect C-erbB-2 protein and mRNA expression levels. CONCLUSION: The oncogene C-erbB-2 plays an important role in carcinogenesis, development and CT signs of lung cancer. Detection of this gene combined with CT signs can be used as an effective indicator for clinical diagnosis and assessment of prognosis.