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目的研究局灶低温及全脑低温治疗对SD大鼠创伤性脑损伤后脑水含量、Na+含量及IL-1β、TNF-α的影响,以探讨局灶低温对创伤性脑损伤的治疗作用及可能机制。方法采用自由落体打击装置造成大鼠创伤性脑损伤,分别于伤后30min开始进行全脑降温、25℃水脑局灶降温治疗,治疗结束后在相应时相点断头处死,采用相关的方法测定伤灶脑组织含量,Na+含量、白细胞介素-1β(IL-1β)和肿瘤坏死因子(TNF-α)活性含量和IL-1β和TNF-αmRNA表达等。结果在含水量方面、在离子含量方面、在IL-1β和TNF-α含量方面以及在IL-1β和TNF-αmRNA表达方面,局灶低温(25℃)组与全脑低温组以及脑外伤模型组相比均呈现不同程度的变化。结论局灶低温(25℃)治疗能明显减轻脑水肿;全脑低温治疗加重脑水肿;其机制之一可能是通过抑制或促进炎性因子IL-1β、TNF-α表达而起作用。
Objective To study the effects of focal hypothermia and whole-brain hypothermia on brain water content, Na + content, IL-1β and TNF-α in traumatic brain injury of SD rats and to explore the therapeutic effect of focal hypothermia on traumatic brain injury mechanism. Methods Traumatic brain injury was induced by free-fall strike in rats. Whole brain cooling was started at 30 min after injury and focal cerebral hydrocephalus was warmed at 25 ℃. After treatment, the animals were decapitated at the corresponding time points. The relevant methods The contents of brain tissue, Na +, interleukin-1β (IL-1β) and tumor necrosis factor (TNF-α) and IL-1β and TNF- Results In terms of water content, in terms of ion content, in terms of IL-1β and TNF-α content as well as IL-1β and TNF-αmRNA expression, focal low temperature (25 ℃) group and whole brain hypothermia group, Compared to the group showed varying degrees of change. Conclusions Localized hypothermia (25 ℃) can significantly reduce cerebral edema. Whole brain hypothermia may aggravate cerebral edema. One of the mechanisms may be through inhibiting or promoting the expression of inflammatory cytokines IL-1β and TNF-α.