论文部分内容阅读
目的:观察壮骨止痛方无水乙醇提取A部位对去势雌鼠骨质松模型的疗效,并探讨其可能的作用机制。方法:选取250 g左右SD雌性大鼠75只,按体重随机分为模型组、假手术组、正常组、壮骨止痛方无水乙醇提取A部位组(0.12 g.kg-1.d-1)、壮骨止痛方组(13.2 g.kg-1.d-1),每组15只。去卵巢术后5 d开始给药,连续13周。取血和骨标本从骨密度、骨生物力学、病理形态学等几个方面研究其药效,从血清雌激素(E2)、骨钙素(BGP)、甲状旁腺素(PTH)、降钙素(CT)方面研究其作用机制。结果:壮骨止痛方无水乙醇提取A部位能明显增加去卵巢模型鼠股骨、腰椎骨的骨密度(P<0.05);显著提高股骨头平均骨小梁密度(P<0.01);显著增强模型大鼠胫骨的最大应力和最大抗弯强度(P<0.01);与去势模型组比较壮骨止痛方无水乙醇提取A部位明显增加子宫系数(P<0.05),能显著降低去势模型鼠血清CT(P<0.01)。结论:壮骨止痛方无水乙醇提取A部位能增加模型鼠骨密度及骨最大应力和最大抗弯强度,逆转骨质疏松症的病理形态学改变。其作用机制之一可能是发挥药物自身的雌激素样作用,改善激素代谢紊乱,直接或间接调节了机体的骨代谢和细胞因子,降低了骨转换,促进骨形成,抑制骨吸收。
OBJECTIVE: To observe the curative effect of Zhuanggushi Tongfang decoction on the osteoporosis model of ovariectomized female rats and to explore its possible mechanism. Methods: Seventy-five SD female rats weighing 250 g were randomly divided into model group, sham operation group, normal group and A group (0.12 g.kg-1.d-1 ), Zhuanggu Zhitong Prescription (13.2 g.kg-1.d-1), 15 rats in each group. Ovarian administration began 5 days after surgery for 13 weeks. The blood and bone samples were studied for their effects on bone mineral density, bone biomechanics and pathomorphology. The effects of serum estradiol (E2), BGP, parathyroid hormone (PTH), calcium lowering (CT) aspects of its mechanism of action. Results: Part A of ethanol extract from Zhuanggushi Tongfang could significantly increase the BMD of the femur and lumbar vertebrae in ovariectomized rats (P <0.05), significantly increase the average trabecular bone density of the femoral head (P <0.01), significantly enhance the model (P <0.01) .Compared with the castrated model group, the part of ethanol extract of Zhuangguzhitong decoction significantly increased the uterine coefficient (P <0.05), and could significantly reduce the weight loss of castrated rat Serum CT (P <0.01). Conclusion: Part A of ethanol extract from Zhuanggu Zhitong Recipe can increase bone mineral density, maximal bone stress and maximal flexural strength in model rats and reverse the pathological changes of osteoporosis. One of its mechanism may be to exert the estrogen-like effect of the drug itself, to improve hormone metabolism disorders, directly or indirectly regulate the body’s bone metabolism and cytokines, reduce bone turnover, promote bone formation and inhibit bone resorption.