用荧光分光光度法测定了六种有机药物中的微量硒。样品用氧瓶燃烧法破坏,利用2,3-二氨基萘和硒(Ⅳ)的络合反应,用荧光分光光度计测定其荧光强度。本法的最低检出限为0.0476μg,平均回收率为100.0±3.0%(置信率95%),变异系数小于6%。含硒量在0～2μg/5ml符合线性关系。本法灵敏度高,样品用量少,误差较小,可满足有机药物中微量硒测定的要求。 Determination of Trace Selenium in Six Organic Medicines by Fluorescence Spectrophotometry. The samples were destroyed by the oxygen flask combustion method, and the fluorescence intensity was measured by a fluorescence spectrophotometer using the complexation reaction of 2,3-diaminonaphthalene and selenium (Ⅳ). The minimum detectable limit of this method is 0.0476 μg with an average recovery of 100.0 ± 3.0% (confidence 95%) and a coefficient of variation of less than 6%. Selenium content in 0 ~ 2μg / 5ml in line with the linear relationship. This method has high sensitivity, less sample usage and less error, which can meet the requirements of trace selenium determination in organic medicine.