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从成都动物园因腹泻死亡的凹甲陆龟体内分离到一株病源菌,经选择性分离培养、生化试验、血清型鉴定等,确定该病源菌为D群肠炎沙门氏菌,其抗原结构式为1,9,12∶g,m∶-。该菌株对小鼠有较强的致病性,能引起人工感染小鼠大量死亡,且从其体内分离到相同特性的菌株;药物敏感性试验证实该分离菌对头孢曲松、氧氟沙星、卡拉霉素、多粘菌素、复方新诺明、呋喃唑酮等敏感,对链霉素、四环素、氯霉素等耐药;同时根据GenBank中已报道的沙门氏菌毒力因子ivnA和ivnE的基因序列,设计两对特异性引物对该菌进行PCR检测,结果在该菌中成功扩增并检测出ivnA和ivnE基因。本试验尝试了沙门氏菌的PCR检测方法,为该分子诊断技术的全面推广奠定了良好基础,也为今后该疾病的预防、诊断和治疗提供了科学的实验数据。
The pathogen was identified as Salmonella enteritidis group D in the zoo of Chengdu, due to the isolation of a pathogen from the body of Diurnal Turtle dead in Diarrhea. The antigenic structure was identified as 1, 9 , 12:g, m :-. The strain has a strong pathogenicity in mice, can cause a large number of artificial infection in mice died, and isolates from the same strain isolated from the body; drug sensitivity test confirmed that the isolation of ceftriaxone, ofloxacin , Kamycin, polymyxin, cotrimoxazole, furazolidone and other sensitive to streptomycin, tetracycline, chloramphenicol and other resistance; at the same time according to the GenBank has been reported in virulence factors ivnA and ivnE gene sequence Two pairs of specific primers were designed for PCR detection of this bacterium. As a result, the ivnA and ivnE genes were successfully amplified in this bacterium. This experiment tried the PCR detection method of Salmonella, laid a good foundation for the comprehensive promotion of the molecular diagnostic technology, and provided scientific experimental data for the prevention, diagnosis and treatment of the disease in the future.