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目的观察硬质干酪对大鼠骨质疏松的防护作用。方法 SD大鼠48只,随机分为正常对照组、模型组、干酪高、中、低剂量组和葡萄糖酸钙对照组,每组各8只。除正常组外,其余各组用维甲酸按80mg/(kg.d)连续灌胃建立骨质疏松模型,并灌服相应的受试物和药物,正常组和模型组灌胃给予同等体积蒸馏水。实验结束后处死大鼠,观察骨密度、血清生化、骨矿含量和骨形态计量学的变化。结果模型组左侧股骨骨密度、骨矿含量、骨小梁面积百分比和平均骨小梁宽度均明显减少。血清钙、磷、碱性磷酸酶活性、抗酒石酸酸性磷酸酶含量和破骨细胞均明显升高,提示骨质疏松模型复制成功。干酪高剂量组左侧股骨密度、骨矿含量、抗酒石酸酸性磷酸酶含量与正常对照组、葡萄糖酸钙组比较无明显差异。结论通过补充硬质干酪增加钙的摄入,可提高大鼠的骨密度、骨钙、磷含量,对骨质疏松有一定的预防保护作用。
Objective To observe the protective effect of hard cheese on osteoporosis in rats. Methods Forty-eight Sprague-Dawley rats were randomly divided into normal control group, model group, high, middle and low dose of cheese group and calcium gluconate control group, with 8 rats in each group. In addition to the normal group, the remaining groups with the retinoic acid 80mg / (kg.d) continuous gavage to establish the model of osteoporosis, and fed the appropriate test substance and drug, normal group and model group by intragastric administration of the same volume of distilled water . After the experiment, the rats were sacrificed and the changes of BMD, serum biochemistry, bone mineral content and bone morphometry were observed. Results Left femur BMD, bone mineral content, trabecular area percentage and average trabecular width were significantly decreased in the model group. Serum calcium, phosphorus, alkaline phosphatase activity, tartrate-resistant acid phosphatase and osteoclasts were significantly increased, suggesting that osteoporosis model replication success. There was no significant difference in left femur density, bone mineral content and tartrate-resistant acid phosphatase content between high-dose cheese group and normal control group and calcium gluconate group. Conclusion Supplementation of hard cheese to increase calcium intake can improve bone mineral density, bone calcium and phosphorus content in rats, and have some preventive and protective effects on osteoporosis.