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目的:建立10批不同产地川芎药材多波长覆盖融合指纹图谱,并对共有峰进行初步指认,通过指纹图谱比较不同产地川芎药材的差异性,为其质量控制和药材鉴别提供依据。方法:采用超高液相色谱法建立不同产地川芎药材的指纹图谱;采用全时段多波长融合技术对dif数据进行多波长融合;采用Spss 19.0数据统计软件,对不同产地川芎药材的共有峰进行聚类分析,区别其差异;采用Q-TOF-MS法对指纹图谱中的共有峰进行指认。结果:建立了川芎药材的多波长融合指纹图谱,确定了20个共有峰并对其中的8个峰进行了指认,通过聚类分析和相似度评价可知云南川芎与其他产地的川芎相比存在着一定的差异性。结论:该指纹图谱重复性良好,能够区分不同产地川芎药材,可作为川芎药材质量评价的一种手段。
OBJECTIVE: To establish multi-wavelength coverage fusion fingerprints of 10 medicinal materials of Chuanxiong rhizome from different areas and preliminary identification of the common peaks. The differences of Chuanxiong medicinal materials in different areas were compared by fingerprints to provide basis for their quality control and identification of medicinal materials. Methods: Fingerprints of Chuanxiong rhizome from different areas were established by ultra-high performance liquid chromatography (HPLC). Multi-wavelength fusion of dif data was performed by using full-time multi-wavelength fusion technique. Spss 19.0 data statistical software was used to collect the common peaks of Chuanxiong rhizome Class analysis, to distinguish the difference; using Q-TOF-MS method to identify the common peak in the fingerprint. Results: The multi-wavelength fusion fingerprints of Chuanxiong rhizome were established, twenty common peaks were identified and eight of them were identified. Cluster analysis and similarity evaluation showed that there was a significant difference between Chuanxiong and other Chuanxiong A certain difference. Conclusion: The fingerprints are reproducible and can distinguish Rhizoma Chuanxiong from different areas, which can be used as a means to evaluate the quality of Rhizoma Chuanxiong.