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目的探讨第10染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)和Ki67蛋白在肺癌组织中的表达及其临床意义。方法采用免疫组化方法检测67例非小细胞肺癌(NSCLC)组织(A组)及41例癌旁正常肺组织(B组)中PTEN基因和Ki67蛋白的表达。结果 A组PTEN基因阳性表达率显著低于B组(32.84%vs.82.93%),而Ki67表达则显著高于B组(76.12%vs.27.31%)(P<0.05)。PTEN基因和Ki67的表达与肺癌的TNM分期、临床分期、淋巴结转移和4年生存率密切相关(P<0.05)。NSCLC的PTEN基因表达与Ki67呈负相关(r=-0.239,P<0.05)。Kaplan-Meier生存曲线分析显示,NSCLC的PTEN表达下调和Ki67表达上调者,术后生存率下降。结论联合检测PTEN与Ki67的表达有助于判断非小细胞肺癌的预后。
Objective To investigate the expression of PTEN and Ki67 on chromosome 10 in lung cancer and its clinical significance. Methods The expressions of PTEN and Ki67 in 67 non-small cell lung cancer (NSCLC) tissues and 41 adjacent normal lung tissues (B) were detected by immunohistochemistry. Results The positive expression rate of PTEN gene in group A was significantly lower than that in group B (32.84% vs.82.93%), while Ki67 expression was significantly higher than that in group B (76.12% vs.27.31%) (P <0.05). The expressions of PTEN and Ki67 were closely related to TNM staging, clinical stage, lymph node metastasis and 4-year survival rate of lung cancer (P <0.05). NSCLC PTEN gene expression and Ki67 was negatively correlated (r = -0.239, P <0.05). Kaplan-Meier survival curve analysis showed that downregulation of PTEN expression and up-regulation of Ki67 expression in NSCLC patients, postoperative survival decreased. Conclusions The combined detection of PTEN and Ki67 expression is helpful to evaluate the prognosis of non-small cell lung cancer.