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目的 探讨T,S细胞异常活化在外源性哮喘免疫调节机制中的作用。方法 对30例外源性哮喘患者发作期和缓解期及30例健康成年人的外周血,采用ELISA双抗体夹心法,测定血清可溶性白介素-2受体(Soluble interleukin-2 receptor,sIL-2R)和白介素-4(Interleukin-4,IL-4)水平,用抗人CD_(23)的McAb测定CD_(23)。结果 外源性哮喘发作期血清sIL-2R,IL-4和CD_(23)水平明显升高,与缓解期和对照组有明显差异(P均<0.01),且CD_(23)水平分别与血清sIL-2R和IL-4之间有明显正相关(r=0.85,0.64,P均<0.01)。结论 T,B细胞异常活化是外源性哮喘发病的关键,通过测定细胞因子水平的变化,监测T,B细胞的活化,为今后临床干预哮喘的发生和治疗提供理论基础。
Objective To investigate the role of abnormal activation of T and S cells in immune regulation mechanism of extrinsic asthma. Methods Serum levels of soluble interleukin-2 receptor (sIL-2R), interleukin-2 receptor (IL-2R) and interleukin-2 receptor were measured by ELISA double antibody sandwich ELISA in 30 exacerbations and exacerbations of extrinsic asthma patients and 30 healthy adults. Interleukin-4 (IL-4) levels were measured, and CD_ (23) was determined using McAbs against human CD_ (23). Results Serum levels of sIL-2R, IL-4 and CD_ (23) in exacerbation group were significantly higher than those in remission stage and control group (all P <0.01), and the levels of CD_ (23) There was a significant positive correlation between sIL-2R and IL-4 (r = 0.85,0.64, P <0.01). Conclusion Abnormal activation of T and B cells is the key to the development of exogenous asthma. Monitoring the activation of T and B cells by measuring the changes of cytokines level provides a theoretical basis for the future clinical intervention in the pathogenesis and treatment of asthma.