采用垂直淀粉胶电泳对中华鳖脑、眼、心、肌、肝、肾和卵巢七种组织中的六种同工酶系统（ＬＤＨ、ＡＤＨ、ＩＤＨ、ＭＥ、Ｇ６ＰＤＨ、ＥＳＴ）进行研究，构建了中华鳖同工酶酶谱，并利用草鱼ＬＤＨ－Ｂ４抗体对中华鳖ＬＤＨ酶谱进行了免疫化学分析，确定了中华鳖ＬＤＨ同工酶酶谱和亚基组成，揭示了鱼类ＬＤＨ与爬行类ＬＤＨ在进化上的保守性 Six isozymes (LDH, ADH, IDH, ME, G6PDH, EST) in seven tissues of the brain, eye, heart, muscle, liver, kidney and ovary of the soft-shelled turtle were studied by vertical starch gel electrophoresis. Chinese turtle isoenzyme zymogram, LDH-B4 antibody was used to detect the LDH isozymes of soft-shelled turtle, and the LDH isozymes and subunit composition of the soft-shelled turtle, LDH is evolutionarily conservative