论文部分内容阅读
目的 :探讨磷酸萘酚喹及其复方的抗疟作用机制。方法 :采用流式细胞术 (FCM)分析了磷酸萘酚喹、青蒿素及两者组成的复方对鼠伯氏疟原虫K173株DNA含量及溶酶体pH值的影响。结果 :复方磷酸萘酚喹和青蒿素均可以使原虫寄生率迅速下降 ,但两者对原虫DNA含量的影响均不明显。磷酸萘酚喹单药给药后 1h疟原虫溶酶体pH值开始升高 ,药后 2h升至最高 ,到 4h基本恢复至药前水平 ;青蒿素对疟原虫溶酶体pH值的影响是先降后升 ;复方磷酸萘酚喹给药后溶酶体pH值变化类似青蒿素 ,但作用较弱。结论 :复方磷酸萘酚喹和青蒿素的杀虫机制与其对疟原虫DNA及疟原虫溶酶体pH值的影响不相关
Objective: To investigate the antimalarial mechanism of naphthoquine phosphate and its compound. Methods: The DNA content and lysosomal pH of parathytina berghei K173 strain were analyzed by flow cytometry (FCM). Results: Both compound naphthoquine and artemisinin could reduce the parasitic rate of protozoa rapidly, but both of them had no obvious effect on DNA content of protozoa. Phospholipase naphthoquinone 1h after administration of plasmin lysosomal pH began to rise, 2h after the drug rose to the highest, to 4h basic restored to pre-drug levels; artemisinin on Plasmodium lysosomal pH value Is the first drop after rising; compound naphthoquine quinoline administration after administration of lysosome pH change similar to artemisinin, but the role of weak. CONCLUSION: The insecticidal mechanism of compound naphthoquine and artemisinin is not related to the effect on the pH of Plasmodium DNA and Plasmodium lysosome