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白介素8(IL-8)是一种强效的中性白细胞趋化性肽,但其对人类趋化活性的作用迄今未明,为了探讨炎性胸液中IL-8的浓度及其对中性白细胞的影响,采用酶联免疫吸附试验(ELISA)检测了脓胸患者胸液中的IL-8和IL-8诱生剂肿瘤坏死因子-α(TNF-α)的浓度和生物活性。共采集51例(诊断包括脓胸14例,类肺炎4例,结核病8例,恶性肿瘤9例,其它渗出液7例,漏出液9例)患者(男43、女8例;年龄49±17岁)的胸液。计数胸液的中性白细胞,并检测上清液中IL-8和TNF-α的浓度。为确定IL-8对脓胸中性白细胞趋化活性的影响,还检测了加入抗IL-8抗体F(ab’)_2段或抗IL-6抗体F(ab’)_2(对照)前后脓性胸液诱导中性白细胞的游走情况。
Interleukin 8 (IL-8) is a potent neutralizing leukocyte chemotactic peptide, but its effect on human chemotactic activity has not been known so far. In order to investigate the concentration of IL-8 in inflammatory pleural effusion and its effect on neutral Leukocyte, the concentration and biological activity of IL-8 and IL-8 inducing agent TNF-α in pleural fluid of empyema patients were detected by enzyme-linked immunosorbent assay (ELISA). A total of 51 patients (43 males and 8 females; age 49 ±, including 14 empyemas, 4 pneumonia-like cases, 8 tuberculosis, 9 malignant tumors, 7 other exudates, and 9 leakage cases) 17 years old) of the pleural fluid. Neutrophils in the pleural fluid were counted and the concentrations of IL-8 and TNF-α in the supernatant were measured. To determine the effect of IL-8 on the chemotactic activity of empyemated neutrophils, the purulence was also assayed before and after the addition of anti-IL-8 antibody F (ab ’) _2 or anti-IL6 antibody F (ab’) _2 Pleural fluid to induce neutrophil migration situation.