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通过实验筛选出了适于特异性的同步检测酸式和内酯式莫奈呵啉K的薄层色谱法 (TLC) .最佳薄层色谱条件为 :薄层板采用德国Merck公司的化学修饰薄层板SilicaGel 6 0 ,RP - 18F2 5 4,0 .5mm ;展开剂系统采用V(氯仿 )∶V(甲醇 )∶V(氨水 ) =2 5∶3∶1;炭化试剂采用 30 % (体积分数 )的硫酸乙醇溶液 ;紫外检测波长为 36 5nm .该法实现了酸式和内脂式莫奈呵啉K的检测特异性与同步性 ,并将检测灵敏度提高到绝对检测量 30ng .该法适用于任何含莫奈呵啉K的样品的检测 ,特别适用于大批量经常性的定性或半定量莫奈呵啉K的检测 .
Through the experiment, thin-layer chromatography (TLC), which is suitable for specific and simultaneous detection of acid and lactone monatin, was screened out.The optimal TLC conditions were as follows: The TLC plate was chemically modified by Merck The development of the system using V (chloroform): V (methanol): V (ammonia) = 25: 3: 1; carbonization reagent using 30% (volume Fraction) of the sulfuric acid ethanol solution; UV detection wavelength of 36 5nm. This method to achieve the acid-type and liposoluble monensin K detection specificity and synchronization, and detection sensitivity increased to the absolute detection of 30ng. It is suitable for the detection of any sample containing Monaramine K and is particularly suitable for the detection of large quantities of regular or semi-quantitative monatin.