论文部分内容阅读
目的观察山柰酚对2型糖尿病大鼠慢性并发症相关因子的影响。方法通过高脂饲料喂养,并ip 30 mg/kg链脲佐菌素(STZ)诱导制备2型糖尿病大鼠模型。山柰酚给药组分别ig给予50、100、200 mg/kg药物,并设模型组、对照组和二甲双胍(0.2 g/kg)阳性对照组。给药10周后,采用葡萄糖氧化酶法对各组大鼠血糖进行测定;放射免疫分析法进行血清胰岛素(INS)的检测,并计算胰岛素敏感指数(ISI);应用酶法对血清中三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)、醛糖还原酶(AR)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TN下-α)的量进行测定;用硫代巴比妥酸(TBA)法检测丙二醛(MDA)的量。结果与对照组比较,模型组大鼠血糖及血清INS水平显著升高(P<0.01),ISI显著降低(P<0.05):模型组大鼠血清TG和LDL-C水平明显升高(P<0.01),HDL-C水平显著降低(P<0.01);模型组大鼠血清MDA、AR、IL-6和TNF-α水平显著升高(P<0.01),SOD活性显著降低(P<0.01)。与模型组比较,山柰酚给药组可降低大鼠血糖水平(P<0.05、0.01),改善胰岛素抵抗,恢复血脂至正常水平,并伴随着MDA、AR、TNF-α、IL-6水平降低(P<0.05、0.01),SOD活性升高(P<0.05、0.01)。结论山柰酚可能是通过降低血糖、改善ISI,维持正常血脂水平,通过抗氧化、抗炎、降低AR途径,从而对2型糖尿病大鼠的慢性并发症起到保护作用。
Objective To observe the effect of kaempferol on chronic complications in type 2 diabetic rats. Methods The rat model of type 2 diabetes was induced by high fat diet and induced by streptozotocin (ip) 30 mg / kg. The sham phenol group were given ig 50,100,200 mg / kg ig, and the model group, the control group and metformin (0.2 g / kg) positive control group. After 10 weeks of administration, the blood glucose of rats in each group was measured by glucose oxidase method; the serum insulin (INS) was measured by radioimmunoassay and the insulin sensitivity index (ISI) was calculated; TG, HDL-C, LDL-C, SOD, AR, IL-6 IL-6) and tumor necrosis factor-α (TN-α). The amount of malondialdehyde (MDA) was detected by thiobarbituric acid (TBA) method. Results Compared with the control group, the levels of serum glucose and serum INS were significantly increased (P <0.01) and the ISI was significantly decreased in model group (P <0.05). The levels of serum TG and LDL-C in model group were significantly increased (P < (P <0.01). The level of serum HDL-C in model group was significantly lower than that in model group (P <0.01). The level of serum MDA, AR, IL-6 and TNF- . Compared with the model group, the kaempferol administration group could reduce the blood glucose level (P <0.05, 0.01), improve the insulin resistance, restore the blood lipid to the normal level, accompanied by the MDA, AR, TNF-α and IL-6 (P <0.05, 0.01), SOD activity increased (P <0.05,0.01). Conclusion The kaempferol may protect the chronic complications of type 2 diabetic rats through decreasing blood glucose, improving ISI, maintaining normal blood lipid levels, and through anti-oxidation, anti-inflammatory and reducing AR pathways.