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目的探讨脂蛋白脂酶活化剂NO_1886对糖尿病小型猪心肌组织血管内皮生长因子(VEGF)蛋白表达的影响。方法15只广西巴马小型猪按体重随机均分3组:正常对照组(CD)饲以正常饲料;高脂高糖高胆固醇组(HFSCD)饲以高脂高糖高胆固醇饲料;加药组(HFSCD+NO_1886),HFSCD饲料中加入1·0%NO_1886。每月末测定血糖、血脂和胰岛素水平。第5个月末取部分心尖组织,HE染色,免疫组化方法检测VEGF的蛋白表达。结果与CD组比较,HFSCD组血糖、高密度脂蛋白胆固醇(HDL_c)、甘油三酯和胰岛素水平异常升高;而HFSCD+NO_1886组与HFSCD组相比,血浆HDL_c显著升高,血糖、甘油三酯和胰岛素明显下降。三组心肌组织VEGF表达的平均积分光密度值分别为:CD0·2798±0·0794;HFSCD0·4394±0·0399;HFSCD+NO_18860·3333±0·0773(P=0·004)。结论NO_1886可降低HFSCD诱导的糖尿病小型猪血糖、甘油三酯和胰岛素,升高HDL_c水平,改善糖代谢和脂代谢紊乱,并对心肌组织VEGF的蛋白表达具有下调作用。
Objective To investigate the effect of lipoprotein lipase activator NO_1886 on the expression of vascular endothelial growth factor (VEGF) in diabetic porcine myocardium. Methods Fifteen Guangxi Bama miniature pigs were randomly divided into three groups according to body weight: normal control group (CD) fed with normal diet, high fat, high glucose and high cholesterol group (HFSCD) fed with high fat, (HFSCD + NO_1886), HFSCD feed added 1.0% NO_1886. Blood glucose, lipids and insulin levels were measured at the end of each month. At the end of the fifth month, some apical tissues were taken, HE staining and immunohistochemistry were used to detect the protein expression of VEGF. Results Compared with CD group, the levels of HDL-C, triglyceride and insulin in HFSCD group were abnormally increased. Compared with HFSCD group, HDL-c in plasma was significantly increased in HFSCD + NO_1886 group, Esters and insulin decreased significantly. The average integral optical density of VEGF expression in three groups of myocardium were: CD0 · 2798 ± 0 · 0794; HFSCD0 · 4394 ± 0 · 0399; HFSCD + NO_18860 · 3333 ± 0 · 0773 (P = 0.004). Conclusion NO_1886 can reduce blood glucose, triglyceride and insulin, increase HDL_c, improve glucose metabolism and lipid metabolism in HFSCD-induced diabetic miniature pigs, and down-regulate the protein expression of VEGF in myocardial tissues.