,Construction of a high-efficiency gene-targeting system in brewing-wine Aspergillus oryzae industri

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Aspergillus oryzae is an important industrial filamentous fungus widely applied in food processing and fermentation industry, and has been safely used for more than 1000 years. For an industrial brewing-wine strain A. oryzae, besides amylase and glucoamylase, it also secretes large amounts of xylanolytic enzymes, such as xylanase (EC 3.2.1.8),β-xylosidase (EC 3.2.1.37),and a-L-arabinofuranosi-dase (L-AF; EC 3.2.1.55), to degrade xylan (in hemicellulose) into D-xylose [1]. In addition, it contains intracellular xylose-utilizing enzymes such as xylose reductase (XR; EC 1.1.1.21) and xylitol dehydrogenase (XDHA; EC 1.1.1.9) to transform D-xylose into useful xylitol [1]. Therefore, A. oryzae can simultaneously saccharify and ferment xylan to xylitol without the addition of xylanolytic enzymes, namely, a new and highly efficient xylitol consolidated bioprocessing (CBP).
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