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目的:观察芒果苷对痤疮主要致病菌的抑菌作用,探讨黄酮类化合物与大环内酯类抗生素的协同抑菌作用机制。方法:采用液体试管法培养金黄色葡萄球菌及痤疮丙酸杆菌,按试管称释法调整细菌浓度,观察芒果苷的抑菌作用。结果:单药使用时,芒果苷对痤疮丙酸杆菌及金黄色葡萄球菌的MIC分别为3 378.4,8 446.0mg.L-1,抑菌效果不明显;当与红霉素联合使用时,降低了红霉素的使用量,浓度为42.23mg.L-1的芒果苷与0.117mg.L-1的红霉素配伍使用,对上述两致病菌有抑制作用。结论:黄酮类化合物与大环内酯类抗生素具有协同抑菌作用,其作用机制极有可能是两者化学结构具有互补性,黄酮类化合物氧杂蒽酮C-葡萄糖苷的部分糖性基因在大环内酯类抗生素的大环内酯环上起到了修饰基团样的作用,由此,有望成为药物工业生产抗菌新药的根据。
Objective: To observe the inhibitory effect of mangiferin on the main pathogens of acne and to explore the synergistic antimicrobial mechanism of flavonoids and macrolide antibiotics. Methods: Staphylococcus aureus and Propionibacterium acnes were cultured by liquid test tube method. The concentration of bacteria was adjusted according to the test tube method, and the antibacterial activity of mangiferin was observed. Results: The MIC values of mangiferin against P. acnes and S. aureus were 3 378.4 and 8 446.0 mg.L-1, respectively. The antibacterial effect was not obvious. When used in combination with erythromycin, The use of erythromycin, the concentration of 42.23mg.L-1 of mangiferin and 0.117mg.L-1 of erythromycin compatibility, the two pathogenic bacteria inhibitory effect. CONCLUSION: Flavonoids and macrolide antibiotics have synergistic antimicrobial activity. The mechanism of action is most likely that the chemical structures of the two compounds are complementary. The partial glycoconjugation genes of flavonoid xanthone C-glucosidase The macrolide macrolide ring has played a role in modifying the group-like, thus, is expected to become the drug industry’s basis for the production of antibacterial drugs.