We employ finite element method to investigate the kinematics of non-axially positioned vesicles through a pore.To complete the coupling between fluid flow and the vesicle membranes,we use the fluid structure interactions with the arbitrary Lagrangian Eulerian method.Our results demonstrate that the vesicles show different deformations in migration process,in t an oblique ellipse-shape,slipper-shape,oval-shape.We find that the rotation angle of non-axially positioned vesicles mainly shows the trend of increase,besides the small fluctuation induced by deformation relaxation.Moreover,when the vesicles move towards the axis of the channel,the rotation angle exhibits a decrease because of the decrease of the shear force.However,rotation of axially positioned vesicles hardly occur due to symmetrical shear force.Our results further indicate that the rotation is faster nearby the pore for non-axially positioned vesicles.Our work answers the mapping between the positions of vesicles and deformed states,as well as the change of rotation angle and rotation velocity,which can provide helpful information on the utilization of vesicles in pharmaceutical,chemical,and physiological processes.