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作者在研究了IL-1β对人牙周膜纤维细胞(humen periodontal ligament fi broblast,HPLF)DNA合成功能调节作用的基础上,采用核酸杂交,分子探针技术,动态观察了IL-1β(100u/ml)对该细胞的c-myc基因表达的影响,以期从基因水平探讨IL-1β促进人牙周膜纤维细胞DNA合成的作用机理以及IL-1β与尖周病、牙周病发病、修复的相互关系。结果发现,HPLF经IL-1β处理后,c-mgc基因表达信号显著增强。表明IL-1β具有促进HPLF的c-myc基因表达的作用;IL-1β诱导HPLF的DNA合成可能主要作用于细胞分裂的GO/G_1期;IL-1β在尖周病,牙周病的纤维化愈合过程中可能起着一定的积极效应。
On the basis of studying the regulatory effect of IL-1β on DNA synthesis in human periodontal ligament cell wall fibroblasts (HPLFs), the authors used nucleic acid hybridization and molecular probe techniques to dynamically observe the effects of IL-1β (100u / ml) on the c-myc gene expression of the cells in order to explore the role of IL-1β in promoting DNA synthesis of human periodontal ligament fibroblasts at the gene level as well as the role of IL-1β in the pathogenesis and repair of periodontal disease and periodontal disease Interrelationship. The results showed that, after IL-1β treatment, the expression of c-mgc gene was significantly increased. IL-1β could promote the expression of c-myc gene in HPLF; IL-1βinduced HPLF DNA synthesis may mainly play a role in the cell division GO / G_1 phase; IL-1β in apical and periodontal fibrosis Healing process may play a positive effect.