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目的:探讨转移生长因子β1(transforminggrowth factor beta-l,TGF-β1)位点rs200482214基因多态性与黑龙江省汉族人群慢性牙周炎的易感性的相关性。方法:选取2012年3月至2013年7月在哈尔滨医科大学附属口腔医院牙周科就诊的135例轻、中、重度慢性牙周炎汉族患者(牙周炎组)和108例汉族健康对照者(健康对照组)作为研究对象,基因组DNA来自口腔颊粘膜拭子,采用多重单碱基延伸SNP分型技术(Multiplex SNaPshot technique)对所有受试者TGF-β1基因rs200482214位点进行检测,比较两组间此位点基因型分布和等位基因频率的差异。结果:(1)TGF-β1基因rs200482214位点各基因型(GG、GA、AA)分布均符合Hardy-Weinberg遗传平衡定律(P>0.05);(2)TGF-β1基因rs200482214位点GG、GA、AA在牙周炎组和健康对照组的分布频率分别为61.5%、30.4%、8.1%和63.0%、28.7%、8.3%,两组人群基因型分布频率差异无统计学意义(P>0.05);等位基因G、A在牙周炎组和健康对照组分布频率分别为76.7%、23.3%和77.3%、22.7%,两组人群的等位基因分布频率差异亦无统计学意义(P>0.05)。结论:TGF-β1位点rs200482214基因多态性与黑龙江省汉族人群慢性牙周炎的易感性不具有相关性。
Objective: To investigate the association between rs200482214 polymorphism of transforming growth factor β1 (TGF-β1) gene and susceptibility to chronic periodontitis in Han nationality in Heilongjiang Province. Methods: From March 2012 to July 2013, 135 cases of mild, moderate and severe chronic periodontitis (periodontitis group) and 108 healthy control subjects from Han nationality were enrolled in the Department of Periodontology of Harbin Medical University Stomatology Hospital. (Healthy control group) as the research object, the genomic DNA was from the buccal mucosal swabs of the mouth and the rs200482214 site of TGF-β1 gene was detected in all subjects by using Multiplex SNaPshot technique. Differences in genotype distribution and allele frequency at this locus between groups were observed. Results: (1) Distribution of genotypes (GG, GA, AA) of rs200482214 of TGF-β1 gene all accorded with Hardy-Weinberg’s law of genetic balance (P> 0.05). (2) GG, GA of rs200482214 of TGF-β1 gene The distribution frequencies of AA in periodontitis group and healthy control group were 61.5%, 30.4%, 8.1% and 63.0%, 28.7% and 8.3%, respectively. There was no significant difference in genotype frequencies between two groups (P> 0.05 ). The distribution frequency of allele G, A in periodontitis group and healthy control group were 76.7%, 23.3% and 77.3%, 22.7%, respectively. There was no significant difference in allele frequencies between the two groups (P > 0.05). Conclusion: The rs200482214 gene polymorphism at TGF-β1 locus has no correlation with susceptibility to chronic periodontitis in Han nationality in Heilongjiang Province.