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目的建立应用模式生物斑马鱼胚胎进行化学品毒性鉴定的方法。方法将健康亲代成熟斑马鱼进行交配产卵,产卵后收集斑马鱼胚胎,于受精后6 h将斑马鱼胚胎暴露于梯度浓度的甲醛(0、0.005%、0.010%、0.012%、0.014%、0.016%、0.018%、0.020%)、乙醇(0、1.0%、1.2%、1.4%、1.6%、1.8%、2.0%、2.2%、2.4%)、三氯异氰尿酸(0、2μg/mL、4μg/mL、6μg/mL、8μg/mL、10μg/mL、12μg/mL、14μg/mL、16μg/mL、18μg/mL、20μg/mL)、甲醇(0、0.6%、0.9%、1.4%、2.0%、3.0%、4.5%),在暴露后24 h、48 h、72 h、96 h、120 h观察斑马鱼胚胎的死亡情况。结果甲醛、乙醇、三氯异氰尿酸、甲醇暴露后,随着剂量的升高,斑马鱼胚胎在各观察时间点出现死亡明显升高,甲醛、乙醇、三氯异氰尿酸、甲醇对受精后6 h斑马鱼胚胎的96 h LD50分别为0.001 6 mol/L、0.333 0 mol/L、7.514 0μg/m L、0.401 0 mol/L。结论斑马鱼胚胎可用于化学品暴露的安全性评价,应用模式生物斑马鱼胚胎进行化学品毒性鉴定是未来可发展的毒理学替代方法之一。
Objective To establish a method for chemical toxicity identification of zebrafish embryos. Methods Zebrafish embryos of healthy parental matured males were oviposited and oviposited. The zebrafish embryos were exposed to the gradient of formaldehyde (0,0.005%, 0.010%, 0.012%, 0.014% 0.016%, 0.018%, 0.020%), Ethanol (0,1.0%, 1.2%, 1.4%, 1.6%, 1.8%, 2.0%, 2.2%, 2.4%), Trichloroisocyanuric acid (0, 0.6%, 0.9%, 1.4%) at a concentration of 1 mg / , 2.0%, 3.0% and 4.5% respectively). The deaths of zebrafish embryos were observed at 24 h, 48 h, 72 h, 96 h and 120 h after exposure. Results After the exposure of formaldehyde, ethanol, trichloroisocyanuric acid and methanol, the death of zebrafish embryos was significantly increased at each time point after the exposure. After formaldehyde, ethanol, trichloroisocyanuric acid and methanol were fertilized The 96 hour LD50 of 6 h zebrafish embryos were 0.001 6 mol / L, 0.333 0 mol / L, 7.514 0 μg / mL and 0.401 0 mol / L, respectively. Conclusion The zebrafish embryos can be used to evaluate the safety of chemical exposure. It is one of the toxicological alternatives that can be developed in the future using chemical zebrafish embryos.