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以二乙基亚硝胺 (DEN)启动、切除部分肝组织和腹腔注射 CCl4(PH/ CCl4)促进联合作用造成大鼠肝癌癌前病变模型 ,试验组除上述处理外饮用 0 .1%的茶多酚或茶色素 ,8周后宰杀 ,测定肝细胞浆谷胱甘肽硫转移酶 (GST)活性 ,用 GST1- 1、1- 2、3- 3、Pi多克隆抗体 Western Blotting方法检测 GST各亚型蛋白质的相对水平。研究发现茶多酚或茶色素可使 DEN/ PH/ CCl4组动物的 GST活性降低有明显的回升 ;主要表现为茶多酚、茶色素对 GST1- 1、1- 2、3- 3都有不同程度的诱导 ,由于 GST1- 1、GST1- 2属于 GSTα类 ,GST3- 3则属 GSTμ类 ,因此茶多酚主要诱导 GSTα,而茶色素则主要诱导 GSTμ。而对 GST- Pi有明显的抑制作用。本研究表明 ,茶多酚和茶色素都能通过有效调节大鼠体内 相代谢酶 ,从而抑制 DEN诱发的大鼠癌前病变的发生和发展。
The rat liver cancer precancerous lesion model was induced by the combination of diethylnitrosamine (DEN) activation, partial hepatic tissue ablation and intraperitoneal injection of CCl4 (PH/CCl4). The experimental group consumed 0.1% tea in addition to the above treatments. Polyphenols or tea pigments were slaughtered after 8 weeks to determine the activity of glutathione-S-transferase (GST) in liver cytoplasm. GST1-, 1-, 2-, 3-, and Pi polyclonal antibodies were used to detect GST by Western Blotting. The relative level of subtype protein. Studies have found that tea polyphenols or tea pigments can significantly reduce the GST activity in DEN/PH/CCl4 animals; the main manifestations are that tea polyphenols and tea pigments are different for GST1-1, 1-2, and 3-3. The degree of induction, because GST1-1, GST1- 2 belong to GSTα class, GST3- 3 belong to GSTμ class, so tea polyphenols mainly induce GSTα, while tea pigment mainly induces GSTμ. However, GST-Pi has a significant inhibitory effect. This study shows that both tea polyphenols and tea pigments can effectively regulate the metabolic enzymes in rat in vivo, thereby inhibiting the occurrence and development of rat precancerous lesions induced by DEN.