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Objective:To evaluate the 15-lipoxygenase inhibitory activity of the methanol leaf extracts of Commelina benghalensis,Tradescantia fluminensis(T.fluminensis)and Tradescantia zebrina.Method:The inhibitory activity was evaluated using a spectrophotometric assay by observing the increase in absorbance at 234 nm due to the formation of the product 13-hydroperoxyoctadecadienoic acid.The extracts were also tested for the presence of terpenoids,saponins,tannins,flavonoids,steroids,phenolic compounds,alkaloids and cardiac glycosides.Results:All the extracts inhibited the action of 15-lipoxygenase at a concentration of 0.2μg/mL.T.fluminensis and Tradescantia zebrina exhibited higher than 50%inhibition with T.fluminensis at 87.2%.T.fluminensis was partitioned with ethyl acetate and hexane and their IC_(50)values were determined at 8.72μg/mL and 98.04μg/mL,respectively.Conclusions:T.fluminensis is a potentially good source of 15-lipoxygenase inhibitors.
Objective: To evaluate the 15-lipoxygenase inhibitory activity of the methanol leaf extracts of Commelina benghalensis, Tradescantia fluminensis (T. fluminensis) and Tradescantia zebrina. Method: The inhibitory activity was evaluated using a spectrophotometric assay by observing the increase in absorbance at 234 nm due to the formation of the product 13-hydroperoxyoctadecadienoic acid. The extracts were also tested for the presence of terpenoids, saponins, tannins, flavonoids, steroids, phenolic compounds, alkaloids and cardiac glycosides. Results: All the extracts inhibited the action of 15- lipoxygenase at a concentration of 0.2 μg / mL.T.fluminensis and Tradescantia zebrina Exploration higher than 50% inhibition with T.fluminensis at 87.2% .T.fluminensis was partitioned with ethyl acetate and hexane and their IC 50 values were determined at 8.72 μg / mL and 98.04 μg / mL, respectively. Conclusions: T. fluminensis is a potentially good source of 15-lipoxygenase inhibitors.