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目的 通过检测心搏骤停犬复苏后及给予纳洛酮干预后脑组织中S1 0 0蛋白表达情况 ,了解纳洛酮对脑复苏的影响。方法 1 8只健康杂种犬 ,随机分成 3组 ,每组 6只 ,予体外电击诱发室颤 ,对照组 :心搏停后予标准心肺复苏术 ;实验组 :心搏骤停后予标准心肺复苏术 +纳洛酮 ;空白组 :不诱发室颤 ,于复苏后 6h取脑海马组织行脑形态学检查 ,及S1 0 0蛋白表达的测定。结果 实验组S1 0 0蛋白表达明显低于对照组 (P <0 0 1 ) ,实验组脑组织的病理损害低于对照组。结论 使用纳洛酮后心肺复苏犬脑组织的病理损害有所减轻 ,脑组织S1 0 0蛋白的生成也显著减少 ,纳洛酮可能通过减少S1 0 0蛋白的表达而减轻心肺复苏后脑的再灌流损伤
Objective To investigate the effect of naloxone on cerebral resuscitation by detecting the expression of S1 0 0 protein in brain tissue after resuscitation of cardiac arrest dogs and naloxone intervention. Methods A total of 8 healthy mongrel dogs were randomly divided into 3 groups with 6 rats in each group, which were induced by extracorporeal shock (VF). Control group: standard CPR after cardiac arrest; experimental group: standard cardiopulmonary resuscitation after cardiac arrest Surgery + naloxone. In the blank group, ventricular fibrillation was not induced. The brain hippocampus was harvested at 6 hours after resuscitation for morphological examination and S1 0 0 protein expression. Results The S1 0 0 protein expression in the experimental group was significantly lower than that in the control group (P <0.01). The pathological damage in the experimental group was lower than that in the control group. Conclusion The pathological changes of brain tissue after Naloxone treatment are alleviated and the production of S100 protein in brain tissue is also significantly reduced. Naloxone may reduce the reperfusion of brain after cardiopulmonary resuscitation by decreasing the expression of S100 protein damage