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目的:探索不同剂量茶多酚预处理对β-淀粉样多肽(Aβ)所致大鼠学习记忆障碍的影响。方法:将50只健康SD大鼠随机分为假手术组;模型组;茶多酚组(低、中、高3个剂量组)。采用Aβ25-35建立AD模型,给药组造模前2周按低、中、高3种不同剂量灌服茶多酚,造模后再连续灌服2周,每天1次。干预结束后,利用Morris水迷宫实验观察大鼠记忆能力改变。之后处死大鼠,检测各组海马部位Ach、ChAT及AchE的含量或活性。结果:模型组与假手术组相比,大鼠找到平台的时间及穿越站台所在位置的次数明显减少,Ach含量和ChAT活性明显降低,AchE活性有增加趋势,但与假手术组相比,没有显著差异(P>0.05);给予不同剂量的茶多酚能不同程度改善AD大鼠的学习记忆障碍,提高AD大鼠Ach含量和ChAT的活性,与模型组比较有显著性差异,但对AchE活性影响不明显。结论:茶多酚能够改善AD模型大鼠学习记忆能力,提高大鼠脑组织ChAT活性,提示药物的作用可能与胆碱递质合成增加有关。
Objective: To explore the effects of different doses of tea polyphenols preconditioning on learning and memory impairments induced by β-amyloid polypeptide (Aβ) in rats. Methods: Fifty healthy SD rats were randomly divided into sham operation group, model group and tea polyphenols group (low, middle and high dose groups). AD model was established by A|Â25-35, and the polyphenols were infused at three different doses (low, middle and high) two weeks before the model administration. The model rats were continuously fed with polyphenols twice a day for one week. After the intervention, the change of memory ability in rats was observed by Morris water maze test. Afterwards, the rats were sacrificed and the content or activity of Ach, ChAT and AchE in each group were detected. Results: Compared with the sham operation group, the time of locating the platform and crossing the platform in the model group decreased significantly, the Ach content and ChAT activity decreased significantly, while the activity of AchE increased. However, compared with the sham operation group, (P> 0.05). Different doses of tea polyphenols could ameliorate the learning and memory impairment of AD rats to some extent and improve the content of Ach and the activity of ChAT in AD rats. Compared with the model group, The effect of activity is not obvious. CONCLUSION: Tea polyphenols can improve the learning and memory abilities of AD model rats and improve the activity of ChAT in rat brain, suggesting that the effect of the drugs may be related to the increased synthesis of choline neurotransmitters.