【摘 要】
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Dear Editor,Gene editing is a process to introduce desired changes into targeted loci of genomic DNA.Recently,type Ⅱ clustered regularly interspaced short pali
【机 构】
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Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing 100094,
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Dear Editor,Gene editing is a process to introduce desired changes into targeted loci of genomic DNA.Recently,type Ⅱ clustered regularly interspaced short palindromic repeats-associated Cas9 endonuclease (CRISPR/Cas9) system has been demonstrated as a versatile tool for engineering eukaryote genome (Hsu et al.,2014),such as in mice (Zuo et al.,2017).CRISPR/Cas9-mediated genome editing is achieved by the error-prone DNA repair of non-homologous end joining (NHEJ) after double strand DNA cleavage.However,the editing results are unreliable due to uncontrolled random indels.Moreover,it was also occasionally reported that Cas9 may induce troublesome off-target effects (Hsu et al.,2013;Pattanayak et al.,2013;Cho et al.,2014).
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