我们应用直接免疫荧光染色常规福尔马林固定,未经胰酶消化的石蜡切片获得成功,现报告如下。 一、标本收集 收集临床诊断或疑为狂犬病的尸检标本10例,均系常规福尔马林固定,最短7天,最长13年。另收集犬咬伤用狂犬疫苗接种后死亡的临床疑为狂犬疫苗引起播散性脑背髓炎的尸检标本2例。每例均自两侧大脑半球的额叶、顶叶、颞叶、枕叶和海马各取一块,小脑、中脑、桥脑和延髓各取一块,组织 We use direct immunofluorescence staining routine formalin fixed, without the digestion of pancreatic paraffin sections were successful, are as follows. First, the specimens collected Collect clinical examination or suspected rabies autopsy specimens of 10 cases, all regular formalin fixation, the shortest 7 days, up to 13 years. Another collection of dog bites with rabies vaccine inoculation clinical suspicion rabies vaccine caused by disseminated septicemia autopsy specimens in 2 cases. Each case was taken from the frontal lobe, parietal lobe, parietal lobe, temporal lobe, occipital lobe and hippocampus from both sides of the cerebral hemispheres, each taken a piece of the cerebellum, midbrain, pons and medulla oblongata each take a piece of tissue