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为考察铀矿区污染土壤再次受到外源铀胁迫下土壤微生物群落的变化情况,采用Biolog-ECO微平板技术,研究了铀胁迫质量比(100 mg/kg、200 mg/kg)和胁迫时间(7 d、14 d)对铀矿区土壤微生物群落的影响,以原土样为空白对照。结果表明,同一铀胁迫质量比下,延长胁迫时间可增强微生物活性,增加群落均匀度;100 mg/kg铀胁迫下,培养14 d的颜色平均变化率(AWCD)是空白对照的1.4倍,群落多样性明显增加;而200 mg/kg铀胁迫下,微生物活性受到一定的抑制,多样性降低。微生物群落对酚酸类碳源的利用能力随外源铀胁迫质量比增加而降低,对胺类和氨基酸类碳源的利用能力随胁迫时间延长而增强。外源铀胁迫质量比主要影响微生物群落对24种碳源的利用,而胁迫时间主要影响对12种碳源的利用活性。
In order to investigate the change of soil microbial community under uranium stress in uranium mining area again, the Biolog-ECO microplate technique was used to study the effects of uranium stress mass ratio (100 mg / kg, 200 mg / kg) and stress time d, 14 d) on the soil microbial community in uranium mining area, taking the original soil sample as blank control. The results showed that under the same uranium stress, prolonging the stress time could enhance the microbial activity and increase the evenness of the community. Under the 100 mg / kg uranium stress, the average color change rate (AWCD) of 14 d after culture was 1.4 times higher than that of the control The diversity increased obviously. However, under the uranium stress of 200 mg / kg, the microbial activity was inhibited to a certain extent, and the diversity was reduced. The ability of microbial communities to use phenolic acids as source of uranium increased with the increase of uranium stress, while the utilization of amines and amino acids increased with the prolongation of stress time. The ratio of uranium to uranium stress mainly affected the utilization of 24 carbon sources by microbial communities, while the stress time mainly affected the utilization of 12 carbon sources.