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通过RT-PCR方法从玉米中克隆了一个拟南芥LTP3的同源基因,并命名为ZmLTP3。RT-PCR分析发现,ZmLTP3的表达可以被多种生物和非生物胁迫因子所诱导。通过构建植物表达载体,把ZmLTP3的全长cDNA转化到拟南芥中,以野生型为对照,检测转ZmLTP3基因纯合体株系的抗盐性。结果表明,在盐胁迫条件下,转基因拟南芥生长状况较好,植株鲜重、干重、种子产量以及脯氨酸含量显著高于野生型株系,丙二醛含量和外渗电导率显著低于野生型株系,转基因拟南芥提高了抗盐性。
A homologous gene of Arabidopsis LTP3 was cloned from maize by RT-PCR and named ZmLTP3. RT-PCR analysis showed that ZmLTP3 expression can be induced by a variety of biological and abiotic stress factors. The full length cDNA of ZmLTP3 was transformed into Arabidopsis by constructing a plant expression vector. The wild type was used as a control to test the salt resistance of ZmLTP3 homozygous lines. The results showed that the transgenic Arabidopsis grew well under salt stress conditions. The fresh weight, dry weight, seed yield and proline content of transgenic Arabidopsis thaliana were significantly higher than those of wild type strain. The content of MDA and the extravasated conductivity were significantly Lower than the wild-type strain, transgenic Arabidopsis increased salt resistance.