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10名晚期胃癌患者接受此法。4名为根治性手术后复发患者,6名初发病例,其中3人肿瘤直接侵及胰腺并有腹膜播散,另外3名病人分别为腹膜播散、Virchow淋巴结和主动脉旁淋巴结转移及Virchow淋巴结转移、多发性肝转移。 化疗前在硬膜外麻醉下从髂骨采取1000ml肝素化骨髓,离心获得骨髓细胞,将之悬浮于含40％自体血清和10％二甲基亚砜的RPMI-1640中,置于程序冷冻器中以-1℃/每分钟的冷冻速率冷冻后,保存于液氮中。 治疗前一周即做中心静脉插管。治疗前7天开始口服多粘菌素B(1000000单位,每天3次)、万古霉素(1000mg,每天2次),联用磺胺
Ten patients with advanced gastric cancer received this method. Four patients were relapsed after curative surgery. Six primary cases were reported. Three tumors directly invaded the pancreas with peritoneal dissemination. The other three patients had peritoneal dissemination, Virchow lymph node and paraaortic lymph node metastases, and Virchow. Lymph node metastasis, multiple liver metastases. 1000ml of heparinized bone marrow was taken from the iliac bone under epidural anesthesia before chemotherapy. Bone marrow cells were obtained by centrifugation and suspended in RPMI-1640 containing 40% autologous serum and 10% dimethyl sulfoxide and placed in a program freezer. After being frozen at a freezing rate of -1°C/minute, it was stored in liquid nitrogen. One week before the treatment, the central vein was intubated. Oral Polymyxin B (1000000 units, 3 times daily), vancomycin (1000mg, 2 times daily), combined with sulfamethazine 7 days before treatment