Objective:To investigate the roles of COX-2,TNF-α,IL-6 in the pathogenesis of autoimmunetype recurrent spontaneous abortion(RSA).Methods:RT-PCR was used to detect the mRNA of COX-2,TNF-α.IL-6 in the trophoblast cells of murine RSA and normal pregnant models.The COX-2,TNF-α.IL-6 protein expressions were determined by using immunohistochemisry staining method.The COX-2,TNF-α,IL-6 protein expressions were determined by ELISA.Results:The embryo loss rates in experiment group was significantly higher than that in normal pregnancy control group,the expression of COX-2,TNF-α,IL-6 in the trophoblast cells of murine RSA and normal pregnant models.The expression of COX-2 in autoimmunetype recurrent spontaneous abortion was significantly lesser than in normal pregnant models.The expression of TNF-α,IL-6 in autoimmune-type recurrent spontaneous abortion was significantly higher than in normal pregnant models.There was a positively correlation between TNF-αand IL-6.There was no relationship between COX-2,TNF-αand IL-6.Conclusions:The abnormal expression of COX-2,TNF-αand IL-6 may result in RSA. Objective: To investigate the roles of COX-2, TNF-α, IL-6 in the pathogenesis of autoimmune type recurrent spontaneous abortion (RSA). Methods: RT-PCR was used to detect the mRNA of COX-2, TNF- IL-6 in the trophoblast cells of murine RSA and normal pregnant models. COX-2, TNF-α. IL-6 protein transcription were determined by using immunohistochemisry staining method. COX-2, TNF- expressions were determined by ELISA. Results: The embryo loss rates in experiment group was significantly higher than that in normal pregnancy control group, the expression of COX-2, TNF-α, IL-6 in the trophoblast cells of murine RSA and normal pregnant models. The expression of COX-2 in autoimmunetype recurrent spontaneous abortion was significantly lesser than in normal pregnant models. The expression of TNF-alpha, IL-6 in autoimmune-type recurrent spontaneous abortion was significantly higher than in normal pregnant models. was was a positively correlation between TNF-αand IL-6.There was no relationship between COX-2, TNF-αand IL-6.Conclusions: The abnormal expression of COX-2, TNF-αand IL-6 may result in RSA.