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为了建立检测卫氏并殖吸虫抗原的血清学诊断方法,我们制备了针对卫氏并殖吸虫囊蚴和成虫的单克隆抗体,部分鉴定了为单克隆抗体所识别的抗原表位,其中,囊蚴期特异性抗原表位对高碘酸敏感而对蛋白酶具有抵抗力,系碳水化会物;反之,成虫期特异性抗原表位对高碘酸具有抵抗力而对蛋白酶敏感,系多肽。应用Dot-ELISA,以并殖吸虫的期特异性单克隆抗体能够检测人血清中0.3~75ng/ml相应的并殖吸虫抗原。
In order to establish a serological diagnostic method for the detection of Paragonimus westermani antigens, we prepared monoclonal antibodies against Paragonimus westermani and adult worms, and partially identified the epitopes recognized by the monoclonal antibodies, Specific antigenic epitopes of the larvae are sensitive to periodate and are resistant to protease, and are carbohydrate compounds. In contrast, the adult stage specific antigenic epitopes are resistant to periodate and sensitive to protease, and are polypeptides. Dot-ELISA was used to detect the corresponding antigen of Paragonimus in human serum from 0.3 to 75 ng / ml with period-specific monoclonal antibodies against paragonimiasis.