本文采用Sephadex G100柱层析从精浆中制备出男性抑制物(male inhibition material MIM),观察MIM对白细胞介素1(Interleukin 1,IL-1)和白细胞介素2(Interleukin 2,IL-2)的产生及反应性的影响,实验结果表明MIM对IL-1、IL-2产生及反应性的抑制率分别为42.22%、24.23%、72.37及78.71%。提示精浆中MIM可能也通过对IL-1和IL-2的抑制作用,在调节局部或全身免疫应答反应,保护受精卵免遭排斥,维持正常的生育过程中有一定意义。 In this paper, male inhibition material MIM was prepared from seminal plasma by Sephadex G100 column chromatography. The effects of MIM on interleukin 1 (IL-1) and interleukin 2 (IL-2) ) Production and reactivity. The results showed that the inhibitory rates of MIM on the production and reactivity of IL-1 and IL-2 were 42.22%, 24.23%, 72.37 and 78.71%, respectively. These findings suggest that MIM in seminal plasma may also play a role in the normal fertility process by regulating the IL-1 and IL-2 inhibition in local or systemic immune responses and protecting fertilized eggs from rejection.