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目的:探讨小鼠实验性缺氧脑和肺组织丙二醛(Malondialdehyde,MDA)、乳酸(Lactic acid,LD)含量和乳酸脱氢酶(Lactate dehydrogenase,LDH)活性变化。方法:18只小鼠随机分为3组,①正常对照组;②急性缺氧组;③急性一氧化碳染毒组。分别测定脑和肺组织MDA、LD、LDH含量和活性。结果:急性缺氧组和急性一氧化碳染毒组小鼠脑和肺组织MDA和LD含量显著增高。LDH活性在两实验组小鼠肺组织显著降低,而在脑组织无显著性改变。结论:MDA和LD含量在缺氧早期脑和肺组织是易变化性指标,其改变可能与该组织损伤程度有关。肺较脑对缺氧产生的自由基损伤更敏感。
Objective: To investigate the changes of malondialdehyde (MDA), lactate (Lactic acid, LD) and lactate dehydrogenase (LDH) activity in experimental hypoxic brain and lung tissue of mice. Methods: Eighteen mice were randomly divided into three groups: ① normal control group; ② acute hypoxia group; ③ acute carbon monoxide poisoning group. The content and activity of MDA, LD and LDH in brain and lung tissue were determined respectively. Results: The content of MDA and LD in brain and lung tissue of acute hypoxia group and acute carbon monoxide group were significantly increased. LDH activity in the two experimental mice significantly decreased lung tissue, but no significant changes in brain tissue. Conclusions: MDA and LD contents in the early stage of hypoxia are indicators of change in brain and lung tissue, the changes may be related to the degree of tissue damage. Lungs are more sensitive to free radical damage than hypoxia.