论文部分内容阅读
目的调查研究遵义地区孕妇乙型肝炎病毒(HBV)感染及乙肝五项定量,为HBV在母婴传播中的预防提供参考。方法选取2014年1月-2016年6月医院住院孕妇2 648例,以金标法检测HBsAg,HBsAg阳性者采用时间分辨荧光免疫分析技术检测乙肝五项定量,荧光定量PCR法检测HBV-DNA,分析结果。结果 2 648例孕妇中有HBsAg阳性者243例,HBV感染率为9.18%;HBV感染孕妇HBV-DNA检测阳性者134例,阴性109例,阳性构成比为55.14%;HBV感染者以HBsAg+HbeAb+HBcAb阳性者所占比例最高,其次为HBsAg+HBcAb、HBsAg+HBeAg+HBcAb阳性,构成比分别为30.45%、25.93%、17.28%,单纯HBsAg阳性者12例,占4.94%;HBsAg+HBeAg+HBcAb模式42例孕妇HBV-DNA均为阳性,阳性率100.00%,HBsAg+HBeAg、HBsAg+HBeAg+HBeAb+HBcAb、HBsAg+HBeAb、HBsAg+HBcAb、HBsAg+HBeAb+HBcAb、HBsAg+HBsAb模式下HBV-DNA阳性率分别为81.82%、72.73%、71.43%、49.21%、37.84%、8.33%;HBVDNA阳性HBV感染孕妇HBsAg、HBeAg高于HBV-DNA阴性孕妇,HBsAb、HbeAb、HBcAb低于HBV-DNA阴性孕妇,比较差异有统计学意义(P<0.05)。结论遵义地区孕妇中存在一定比例HBV感染,乙肝五项定量能较好反应出HBV复制活跃程度,与HBV-DNA关系密切。
Objective To investigate the hepatitis B virus (HBV) infection in pregnant women in Zunyi and the quantitative determination of hepatitis B in hepatitis B virus (HBV). This study may provide a reference for the prevention of HBV in mother-to-infant transmission. Methods From January 2014 to June 2016, 2 648 pregnant women in hospital were enrolled in this study. Five HBsAg-positive and HBsAg-positive patients were detected by time-resolved fluorescence immunoassay and HBV-DNA were detected by real-time fluorescence quantitative PCR. Analysis results. Results There were 243 cases with positive HBsAg in 2 648 pregnant women and the HBV infection rate was 9.18%. In HBV-infected pregnant women, 134 cases were positive for HBV-DNA test and 109 cases were negative, the positive ratio was 55.14%. The HBsAg + HbeAb + HBcAb positive, followed by HBsAg + HBcAb, HBsAg + HBeAg + HBcAb positive, the constituent ratios were 30.45%, 25.93%, 17.28%, 12 cases were HBsAg positive, accounting for 4.94% The positive rate of HBV-DNA in 42 pregnant women with HBcAb model was 100.00%. HBsAg + HBeAg, HBsAg + HBeAg + HBeAb + HBcAb, HBsAg + HBeAb, HBsAg + HBcAb, HBsAg + HBeAb + The positive rates of HBsAg and HBeAg in HBsAg positive pregnant women were higher than those in HBV-DNA negative pregnant women. The positive rates of HBsAb, HbeAb and HBcAb were lower than that of HBV-DNA Pregnant women, the difference was statistically significant (P <0.05). Conclusion There is a certain proportion of HBV infection in pregnant women in Zunyi area. The five quantitative indexes of HBV can better reflect the activity of HBV replication, which is closely related to HBV-DNA.