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秦巴山是天麻的主产区,由于连年使用旧菌材,导致蜜环菌性状退化,天麻减产。用来分离的材料都是些老菌索,呈黑色、黑褐色,鞘内菌丝黄色干枯,有的呈空壳状。再加上传统的升汞消毒法,组织分离成功率极低。为此,我们对这一方法做了以下改进: 1 按70％的含水量拌好纯木屑培养料,在1只大烧杯底先铺一层料,中间放上老菌材或老菌索,上面再盖一层料,杯口覆塑料薄膜,并扎10个左右的通气小孔,在25℃下培养,20天左右便长出1～2cm长棕红色的生长锥,将它作为分离材料。 2 传统的消毒处理是用升汞,但成功率极低。在生产过程中,我们先取1～1.5cm的生长锥切段用无菌水
Qinba Mountain Gastrodia is the main producing areas, due to years of use of old mushrooms, leading to the degradation of honeycomb fungi, Gastrodia cut. Used to separate the material are old bacteria, black, dark brown, yellow sheath dry mycelium, and some were shell-like. Coupled with the traditional mercuric chloride disinfection, tissue separation success rate is very low. To this end, we have made the following improvements to this method: 1 by 70% water content mix pure wood flour culture material, in a beaker at the end of a layer of material, the middle of the old fungus or old bacteria, And then covered with a layer of material, covered with plastic film cup, and about 10 ventilation holes, cultured at 25 ℃, 20 days or so they grow 1 ~ 2cm long brown red growth cone, it as a separation material . 2 Traditional disinfection uses mercuric chloride, but the success rate is very low. In the production process, we first take 1 ~ 1.5cm growth cone cut with sterile water