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目的:探讨核干细胞因子在食管癌组织中的表达及其与临床病理特征的关系。方法:采用免疫组织化学法检测50例食管癌组织及癌旁组织中核干细胞因子蛋白表达,并分析核干细胞因子与临床病理特征的相关性,随机收集30例食管癌患者的癌组织及癌旁组织,采用荧光定量PCR方法方法检测核干细胞因子mRNA表达情况;结果:免疫组化分析表明,核干细胞因子蛋白在食管癌中阳性表达率为92.0%(46/50)。癌旁组织中核干细胞因子表达阳性率为16.0%(8/50),差异有显著性(P<0.05);核干细胞因子阳性表达率与患者的年龄、性别、肿瘤大小及淋巴结转移无关,与肿瘤的分化程度有关(P<0.05);荧光定量PCR结果显示,食管癌组织中核干细胞因子mRNA表达水平明显高于癌旁正常对照组织。结论:核干细胞因子在食管癌的发生、发展中起着重要作用,可以作为反映食管癌生物学行为的有效指标
Objective: To investigate the expression of nuclear stem cell factor in esophageal cancer and its relationship with clinicopathological features. METHODS: Immunohistochemistry was used to detect the expression of nuclear stem cell factor protein in 50 cases of esophageal cancer tissue and adjacent tissues. The correlation between nuclear stem cell factor and clinicopathological features was analyzed. 30 cases of esophageal cancer tissues and adjacent tissues were randomly collected. Fluorescence quantitative PCR was used to detect the expression of nuclear stem cell factor mRNA. Results: Immunohistochemical analysis showed that the positive expression rate of nuclear stem cell factor protein in esophageal cancer was 92.0% (46/50). The positive rate of nuclear stem cell factor expression was 16.0% (8/50) in adjacent tissues. The difference was statistically significant (P<0.05). The positive expression rate of nuclear stem cell factor was not related to the patient’s age, gender, tumor size and lymph node metastasis. The degree of differentiation was related (P<0.05). The results of fluorescent quantitative PCR showed that the expression level of nuclear stem cell factor mRNA in esophageal cancer tissue was significantly higher than that in normal tissue adjacent to the cancer. Conclusion: Nuclear stem cell factor plays an important role in the occurrence and development of esophageal cancer, and can be used as an effective indicator reflecting the biological behavior of esophageal cancer.