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作者采用Ⅰ型胶原、人羊膜基膜(HABM)基质面、Fibronectin(FN)、多聚鸟氨酸(PO)、PO+FN、PO+Laminin(LN)、鼠尾胶原和 HABM 上皮面作为8天鸡胚前脑细胞生长的支持物,观察这些支持物对前脑细胞生存生长的影响。前脑细胞以每个孔不同的细胞密度在 PO、LN 和HABM 上皮面培养。发现每个孔4×10~5细胞密度细胞生长最好。用 MTT 做为线粒体脱氢酶底物的自动比色法,对前脑细胞在各种支持物上的生长进行了定量测定。结果表明,所测光密度值(OD)与细胞生存、生长成正比,且 HABM 上皮面是前脑细胞生长最好的支持物。各种支持物促进前脑细胞生长的顺序浓次是:HABM 上皮面>鼠尾胶原>PO+LN>PO+FN>PO>FN>HABM 基质面>Ⅰ型胶原。HABM 上皮面比基质面促进前脑细胞生长作用明显强(P<0.01);鼠尾胶原比Ⅰ型胶原强(P<0.05);PO+LN 比 PO 作用强(P<0.05)。这提示 LN 很可能是 HABM、鼠尾胶原和 PO+LN 中促进神经生长的主要活性因子。
The authors used collagen Ⅰ, human amniotic membrane basement membrane (HABM) matrix, Fibronectin (FN), polyornithine (PO), PO + FN, PO + Laminin (LN), murine collagen and HABM epithelial surface as 8 Support of the growth of embryonic chicken embryonic brain cells and the effect of these supports on the survival and growth of forebrain cells were observed. Forebrain cells were cultured on PO, LN and HABM epithelial surfaces at different cell densities per well. It was found that 4 × 10 ~ 5 cell density per well had the best cell growth. Using MTT as an automated colorimetric assay of mitochondrial dehydrogenase substrates, the growth of forebrain cells on various supports was quantitatively determined. The results showed that the measured optical density (OD) is proportional to cell survival and growth, and the HABM epithelial surface is the best supporter of forebrain cell growth. The order of concentrations of various supports for forebrain cell growth was HAPM epithelial> rat tail collagen> PO + LN> PO + FN> PO> FN> HABM matrix> collagen type I. The effect of HABM epithelial cells on forebrain cell proliferation was stronger than that of matrix surface (P <0.01). The collagen of rat tail was stronger than that of collagen type Ⅰ (P <0.05). PO + LN was stronger than PO (P <0.05). This suggests that LN is likely to be the main active factor promoting nerve growth in HABM, murine collagen and PO + LN.