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目的:观察不同剂量黄芪多糖对高脂饮食诱导的大鼠胰岛素抵抗的治疗作用及机制。方法:将成年雄性SD大鼠随机分为正常对照组(NC组,12只)和高脂模型组(HFM组,60只)。模型建立成功后,大鼠随机分组:高脂对照组(HFC组,12只)、吡格列酮组(Pio组,12只)、黄芪多糖(APS)低、中、高剂量组(即LAPS,MAPS,HAPS组,每组均12只),分别给予生理盐水、20 mg·kg-1.d-1的Pio和200,400,800 mg·kg-1.d-1的APS干预8周。随机选取各分组中的一半大鼠进行高胰岛素-正葡萄糖钳夹实验,以稳态期的葡萄糖输注率(GIR)判定周围组织的胰岛素敏感性。同时测定另一半大鼠空腹血糖(FBG),血浆胰岛素(FINS),游离脂肪酸(FFA),血浆脂连素(APN),肿瘤坏死因子(TNF-α)的水平。结果:与NC组比较,HFC组大鼠血浆FINS,FFA,TNF-α的水平显著升高(P<0.05),血浆APN水平和GIR显著降低(P<0.05);与HFC组比较,LAPS,MAPS组相关指标显著改善(P<0.05)。结论:适当剂量的黄芪多糖可以提高肥胖大鼠的胰岛素敏感性,其机制可能与其降低血浆TNF-α,FFA水平和升高APN水平有关。
Objective: To observe the therapeutic effect and mechanism of different doses of astragalus polysaccharides on insulin resistance induced by high-fat diet in rats. Methods: Adult male Sprague-Dawley rats were randomly divided into normal control group (NC group, 12 rats) and high fat model group (HFM group, 60 rats). After successful establishment of the model, rats were randomly divided into four groups: high fat control group (HFC group, 12 rats), pioglitazone group (Pio group, 12 rats), APS low, middle and high dose group HAPS group, 12 rats in each group) were given saline, 20 mg · kg-1.d-1 Pio and 200,400,800 mg · kg-1.d-1 APS for 8 weeks respectively. Half of the rats in each group were randomly selected for hyperinsulinemic-n-glucose clamp test to determine the insulin sensitivity of surrounding tissues with steady-state glucose infusion rate (GIR). The levels of fasting blood glucose (FBG), plasma insulin (FINS), free fatty acid (FFA), plasma adiponectin (APN) and tumor necrosis factor (TNF-α) were measured in the other half of the rats. Results: Compared with NC group, the levels of FINS, FFA and TNF-α in plasma of HFC group were significantly increased (P <0.05) and the level of plasma APN and GIR were significantly decreased (P <0.05). Compared with HFC group, MAPS group related indicators significantly improved (P <0.05). Conclusion: The proper dose of astragalus polysaccharide can improve the insulin sensitivity of obese rats, the mechanism may be related to its lower plasma TNF-α, FFA levels and increased APN levels.