取临床淋巴结活检组织,制成冰冻切片或涂片以抗补体免疫酶标法检测了EB病毒的核抗元(EBNA)。十一例鼻咽癌淋巴结转移标本中,除一例因石蜡切片和冰冻切片诊断不一,EBNA为阴性外,其余10例均为阳性,有些则呈典型的癌巢样分布。鼻咽癌肿瘤细胞以外的各种组织,EBNA均为阴性。此法为鼻咽癌颈淋巴结转移的鉴别诊断提供了辅助性依据。 Clinical lymph node biopsies were taken and frozen sections or smears were made to detect the nuclear antigen (EBNA) of Epstein-Barr virus using anti-complementing immuno-enzyme assay. In eleven cases of nasopharyngeal carcinoma lymph node metastases, except for one case diagnosed by paraffin and frozen sections, EBNA was negative, the remaining 10 cases were all positive, and some had a typical nest-like distribution. All tissues other than nasopharyngeal carcinoma cells were negative for EBNA. This method provides a supplementary basis for the differential diagnosis of cervical lymph node metastasis in nasopharyngeal carcinoma.