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目的 研究细胞增殖及凋亡在宫颈癌中的关系及其临床意义。方法 采用免疫组化技术及DNA缺口末端标记技术对正常宫颈、宫颈上皮内瘤样病变和宫颈鳞癌病人的妇科手术石蜡标本进行细胞增殖和细胞凋亡情况的检测,同时对该组病人的临床及病理资料进行分析。结果 从正常宫颈、CINⅠ-Ⅱ、CINⅢ到宫颈鳞癌,PCNA指数呈增高趋势,凋亡指数和AI/PI呈递减趋势。高PCNA含量较多地出现于恶性程度高及预后倾向差的病例中。高AI与差预后有相关性。高PI、低AI与较高级别的临床分期和病理分级有关,但差异无显著性。结论 CINⅢ、宫颈鳞癌中PI增高和AI、AI/PI比值明显降低,可以协助鉴别 CINⅠ-Ⅱ和CINⅢ,估计肿瘤的恶性潜能。
Objective To study the relationship between cell proliferation and apoptosis in cervical cancer and its clinical significance. Methods Immunohistochemistry and DNA nick end labeling were used to detect cell proliferation and apoptosis in gynecological paraffin-embedded specimens of normal cervix, cervical intraepithelial neoplasia and cervical squamous cell carcinoma patients. Meanwhile, And pathological data analysis. Results From normal cervix, CINⅠ-Ⅱ, CINⅢ to cervical squamous cell carcinoma, the PCNA index showed an increasing trend, and the apoptotic index and AI / PI showed a decreasing trend. High PCNA content appears more in the high degree of malignancy and poor prognosis in cases. High AI and poor prognosis are related. High PI, low AI and higher level of clinical stage and pathological grade, but no significant difference. Conclusion CIN Ⅲ, PI in cervical squamous cell carcinoma and AI, AI / PI ratio decreased significantly, can help identify CIN Ⅰ-Ⅱ and CIN Ⅲ, to estimate the malignant potential of the tumor.