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目的采用HPLC测定复方熊去氧胆酸软胶囊中的硝酸硫胺和维生素B2(Vit B2)。方法色谱柱为Syncronis a Q-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-磷酸盐缓冲液(60∶40),流速1.0 m L·min-1,检测波长273 nm。结果硝酸硫胺81.3~121.9μg·m L-1、Vit B241.0~61.4μg·m L-1与峰面积的线性关系良好(r=0.9999),平均回收率分别为100.3%、100.2%,RSD分别为0.38%、0.68%(n=9)。结论所用方法简便、准确、重复性好,可用于测定复方熊去氧胆酸软胶囊中硝酸硫胺和Vit B2的含量。
Objective To determine thiamine nitrate and vitamin B2 in compound ursodeoxycholic acid soft capsule by HPLC. Methods The mobile phase consisted of Syncronis a Q-C18 column (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-phosphate buffer (60:40) at a flow rate of 1.0 mL · min-1 and a detection wavelength of 273 nm. Results The linear relationship was obtained between 81.3 ~ 121.9 μg · mL -1 thiamine and Vit B241.0 ~ 61.4 μg · mL -1 (r = 0.9999). The average recoveries were 100.3%, 100.2% RSD were 0.38%, 0.68% (n = 9). Conclusion The method is simple, accurate and reproducible. It can be used to determine the content of thiamine nitrate and Vit B2 in compound ursodeoxycholic acid soft capsule.