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目的研究银杏叶提物(EGb)对肝癌细胞生长、甲胎蛋白(AFP)分泌及γ-谷氨酰转移酶(γ-GT)活性的影响。方法以两种人肝癌细胞株SMMC-7721细胞和Bel-7402细胞分别与EGb(17.5～105mg/d)共培养6d,利用MTT比色、ELISA及细胞分化检测等方法。结果Egb(17.5、35、70、105mg/d)均出现细胞增殖抑制,亚细胞结构趋于正常,并且明显降低HepG2细胞高水平分泌AFP。EGb(35、70mg/d)可抑制肝SMMC-7721细胞的γ-GT活性,升高白蛋白(ALB)含量。EGb(70、105mg/d)亦可抑制肝癌Bel-7402细胞的γ-GT活性,并升高ALB含量。结论EGb具有抑制肝癌生长、AFP分泌及γ-GT活性的作用,诱导其在体外向正常肝细胞分化。
Objective To study the effects of Ginkgo biloba extract (EGb) on hepatocellular carcinoma cell growth, AFP secretion and γ-glutamyl transferase (γ-GT) activity. Methods Two human hepatocellular carcinoma cell lines, SMMC-7721 and Bel-7402, were co-cultured with EGb (17.5-105 mg / d) for 6 days respectively. MTT colorimetric assay, ELISA and cell differentiation assay were used. Results Egb (17.5, 35, 70, 105 mg / d) showed inhibition of cell proliferation, subcellular structure tended to be normal, and significantly decreased AFP secretion by HepG2 cells. EGb (35, 70mg / d) inhibited the activity of γ-GT in hepatic SMMC-7721 cells and increased albumin (ALB) content. EGb (70,105 mg / d) also inhibited the γ-GT activity of Bel-7402 hepatoma cells and increased the content of ALB. Conclusion EGb can inhibit the growth of hepatocarcinoma, the secretion of AFP and the activity of γ-GT, and induce its differentiation into normal hepatocytes in vitro.