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从血液学检查诊断α-地中海贫血-2特征常常是不可能的,因为其红细胞形态学和MCV都是正常的。新生儿时期以后,用普通的电泳和层析法亦不能检出Hb Bart′s。本文用免疫学方法证明HbBart′s,是对新生儿期以后的地中海贫血提供了一个诊断性筛选方法。从Hb Bart′s胎儿水肿患者获得Hb Bart′s,反复注入家兔体中使产生抗血清。用双向免疫扩散,免疫电泳和毛细管沉淀试验,证明它仅对Hb Bart′s是特异的。用等体积Hb溶液(2.5～5.0g/dl)和抗Hb Bart′s血清滴入微量血球压积管,试管用聚乙烯塞塞住,4℃或室温放置过夜,管底出现沉淀即为阳性。在Hb Bart′s含量比较多的病例中。于一小时
It is often not possible to diagnose alpha-thalassemia-2 characteristics from hematologic examinations because of their normal erythrocyte morphology and MCV. After neonatal period, Hb Bart’s can not be detected by ordinary electrophoresis and chromatography. This article uses immunological methods to prove that HbBart’s is a diagnostic screening method for thalassemia following the neonatal period. Hb Bart’s were obtained from Hb Bart’s fetal edema and repeatedly injected into rabbits to produce antiserum. Two-way immunodiffusion, immunoelectrophoresis and capillary sedimentation tests demonstrated that it was specific for Hb Bart’s only. Equal volumes of Hb solution (2.5-5.0 g / dl) and anti-Hb Bart’s serum were added to the hematocrit. The test tube was plugged with polyethylene and left overnight at 4 ° C or room temperature. . In more cases of Hb Bart’s content. In an hour