论文部分内容阅读
目的:利用RAPD分子标记技术研究大花红景天的遗传多样性,分析其濒危的可能原因。方法:利用改良的CTAB法提取各产地大花红景天的总DNA,以40条随机引物随机扩增,利用RAPDdistance1.04软件计算多态位点百分率以及遗传距离,并进行聚类分析。结果:大花红景天表现出较为丰富的遗传多样性,40条随机引物筛选出能扩增出清晰稳定条带的12条随机引物。12条随机引物共扩增出99条DNA条带,其中79条表现出多态性,占总带数的79.8%。各产地大花红景天的遗传距离在0.4784-0.7774之间。通过聚类分析可将5个产地的大花红景天分为2个类群。结论:多态位点百分率表明大花红景天具有丰富的遗传多样性,其濒危的可能原因是由于人类无规则且盲目的采挖造成的。
Objective: To study the genetic diversity of Rhodiola rosea by RAPD molecular markers and analyze its possible causes. Methods: The total DNA of Rhodiola sachalinensis was extracted by modified CTAB method. Random amplified polymorphic DNA was amplified with 40 random primers. The percentage of polymorphic loci and genetic distance were calculated by RAPD distance 1.04 software and cluster analysis was performed. Results: Rhodiola sachalinensis showed abundant genetic diversity. Forty random primers were screened for 12 random primers that could amplify clear and stable bands. A total of 99 DNA bands were amplified with 12 random primers, of which 79 showed polymorphism, accounting for 79.8% of the total bands. The origin of large flowers Rhodiola genetic distance between 0.4784-0.7774. Through cluster analysis, Rhodiola rosea in five producing areas can be divided into two groups. Conclusion: The percentage of polymorphic loci indicates that Rhodiola sachalinensis is rich in genetic diversity and its possible endangered cause is due to irregular and blind excavation of human beings.