论文部分内容阅读
对 39名中国人和 1名英国白人α2A 肾上腺素能受体 (α2A AR)全长基因序列分析显示 ,其序列与GenBank中人α2A AR基因序列在调控区、编码区和 3′非编码区存在 13处 g或C插入或缺失的不同 ,但所有人的α2A AR基因序列则完全一致 ,包括该基因不同批次PCR产物正义链和反义链测序结果。得到的人α2A AR基因序列已在GenBank登录 (AY0 32 736 )。对α2A AR基因调控区第 - 12 96位基因型在晕动病易感性不同人群中分布情况的分析结果显示 ,晕动病易感性较高的中国和日本人群分别与易感性较低的英国人群相比 ,前两者该位点 gg基因型分别是后者的 6 .8倍和 8.8倍 ,g等位基因频率均明显高于后者 (P <0 .0 1) ;中国汉族无亲缘关系的晕动病易感人群与不易感人群 (歼击机飞行员 )相比 ,前者该位点 gg基因型是后者的 2 .6倍 ,g等位基因频率亦明显高于后者(P <0 .0 1)。各组人群等位基因分布均符合Hardy Weinberg平衡。提示 ,α2A AR基因第 - 12 96位 gg基因型和g等位基因可能与晕动病易感性有关
The sequence analysis of the full-length α2A adrenergic receptor (α2A AR) from 39 Chinese and one white British white showed that the sequence of the human α2A AR gene in GenBank was located in the regulatory region, coding region and 3 ’non-coding region 13 g or C insertion or deletion of different, but the human α2A AR gene sequence is exactly the same, including the gene of different batches of the PCR product of the sense and antisense strand sequencing results. The resulting human α2A AR gene sequence was registered in GenBank (AY032 736). Analysis of the distribution of the -1296 genotypes in the α2A AR gene regulatory region in different populations susceptible to motion sickness showed that the susceptible populations of Chinese and Japanese with higher susceptibility to motion sickness were associated with the lower susceptibility to the British population Compared with the former two loci gg genotypes were the latter 6.8 times and 8.8 times, g allele frequencies were significantly higher than the latter (P <0.01); unrelated Han Chinese The gg genotype of the former locus was 2.6 times higher than that of the less susceptible population (fighter pilots), and the frequency of the g allele was also significantly higher than that of the latter (P <0. 0 1). The allele distribution of all groups was in line with Hardy Weinberg equilibrium. The results suggest that the -2g to the 96th gg genotype and the g allele of α2A AR gene may be related to susceptibility to motion sickness