在分光光度法中,线性参数的建立是通过计算机,使用最小二乘法计算,从吸收光谱中得到最大可能的信息。在模拟时,标准和样品的吸收光谱用高斯函数来综合,把随机噪音加到光谱中以后,做回归分析,以建立代表标准和样品光谱比例的线性参数。 与测量有关的某些条件,如噪音幅度,波长的再现性及光谱特征,又如半峰宽、光 In spectrophotometry, linear parameters are established by computer using the least-squares method to obtain the maximum possible information from the absorption spectrum. During the simulation, the absorption spectra of the standard and the sample are combined using a Gaussian function. After adding random noise to the spectrum, regression analysis is performed to establish a linear parameter that represents the ratio of the standard to the sample. Some conditions related to the measurement, such as noise amplitude, wavelength reproducibility and spectral characteristics, such as the half-width, light