自从霍洛德尼(Cholodny)50年前提出直接观察土壤中微生物分布的埋片法以来,这种方法曾受到国内外学者很大关注。应用这种方法,对不同土壤、不同土层,或因施肥、耕作等处理的不同,而引起微生物区系组成改变的原位观察,常常获得较为满意的结果。斯梯列(Stille)、斯塔基(Starkey)及林弗尔德(Linfold)等曾用埋片法对一些旱作的根际微生物进行了研究,但对湿饱和的水稻土上生长的水稻根际微生物,尤其 Since Cholodny proposed the direct observation of the distribution of microorganisms in soils 50 years ago, this method has received great attention from domestic and foreign scholars. By applying this method, in situ observation of changes in microbial flora caused by different soils, different soil layers, or due to different treatments such as fertilization and tillage, the satisfactory results are often obtained. Stille, Starkey, and Linfold used to study some of the dryland rhizosphere microbes, but on paddy soils grown on wet-saturated paddy soils Rhizosphere microorganisms, especially