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目的 研究端粒酶逆转录酶基因hTERT在膀胱移行细胞癌 (TCC)中的表达 ,探讨其在TCC发生、发展及预后复发中的意义 .方法 采用兔抗人hTERT多克隆抗体及S P免疫组化法检测在 5 6例TCC及 10例正常膀胱组织中的表达 .结果 hTERT在TCC中阳性表达率为 6 4.3% ,与在正常膀胱组织表达率 0 %相比 ,有显著性差异 (P <0 .0 5 ) .hTERT在Ⅰ ,Ⅱ和Ⅲ级TCC中的表达率分别为 43.8% (7/16 ) ,5 7.7% (15 / 2 6 ) ,10 0 % (14/ 14) .Ⅰ与Ⅲ级、Ⅱ与Ⅲ级、Ⅰ+Ⅱ与Ⅲ级比较差异显著 (P <0 .0 5 ) .hTERT在浅表性及浸润性TCC中的表达率为 5 3.1% (17/ 32 ) ,79.2 % (19/ 2 4) ,两者差异显著 (P <0 .0 5 ) .hTERT阳性表达率与患者复发有显著相关性 (P <0 .0 5 ) .结论 hTERT表达异常可能与膀胱肿瘤的发生、发展有关 .检测hTERT表达可作为预测膀胱肿瘤预后复发的潜在指标
Objective To study the expression of telomerase reverse transcriptase gene hTERT in transitional cell carcinoma of the urinary bladder (TCC) and to explore its significance in the occurrence, development and prognosis of TCC.Methods Rabbit anti-human hTERT polyclonal antibody and SP immunohistochemistry Was detected in 56 cases of TCC and 10 cases of normal bladder tissue.Results The positive expression rate of hTERT in TCC was 6 4.3%, which was significantly different from that in normal bladder tissue (0%) (P <0 .05). The expression rates of hTERT in grade Ⅰ, grade Ⅱ and grade Ⅲ TCC were 43.8% (7/16), 5 7.7% (15/2 6) and 10 0% (14/14) (P <0.05). The expression rates of hTERT in superficial and invasive TCC were 51.1% (17/32), 79.2% (19/24), the difference was significant (P <0.05) .The positive rate of hTERT was significantly correlated with the recurrence (P0.05) .Conclusion The abnormal expression of hTERT may be associated with the occurrence of bladder cancer , The development of detection of hTERT expression can be used as a potential indicator of prognosis of bladder cancer prognosis